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Image Search Results
Journal: Scientific reports
Article Title: GGT1 is a SNP eQTL gene involved in STAT3 activation and associated with the development of Post-ERCP pancreatitis.
doi: 10.1038/s41598-024-60312-2
Figure Lengend Snippet: Figure 1. Five pancreatitis-related genes are positive-regulators for the IL-6 amplifier. (A) H4 cells were transfected with three siRNAs for each gene. qPCR was performed after stimulation with TNF-α plus IL-6. The results of five genes whose siRNA suppressed IL-6 are shown. (B) GGT1 mRNA expression after transfecting H4 cells with three different GGT1-siRNAs and stimulating them with TNF-α plus IL-6. Means ± SEM are shown. *p < 0.05, **p < 0.01, ***p < 0.005; NS, not significant (Student’s t-test).
Article Snippet: After 2 h of serum starvation, 100 ng/mL human IL-6 (Toray), 100 ng/mL soluble IL-6 receptor (IL-6R; PeproTech), and 50 ng/mL human TNF-α (PeproTech) were added to the cells and incubated for 3 h. Total RNA was prepared from the cells using a
Techniques: Transfection, Expressing